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Significant hydrolysis was achieved in just eight h for Hoki roe and 24 h for gemfish roe, further than which a plateau was obtained. Within the initial phase of hydrolysis, the homogenate has an increased volume of protein substrate than in subsequent phases. In the course of this phase, inclined peptide bonds are preferentially hydrolyzed, and the hydrolysis items begin to occupy binding sites which have unhydrolyzed peptide bonds, which results in a discount in the speed of hydrolysis [32,33]. The concentration in the substrate and enzyme is usually a key aspect affecting both of 23hoki those the DH and enough time needed to reach comprehensive hydrolysis [34]. Ahmmed et al. [13] described a whole hydrolysis of Hoki roe homogenate by Alcalase, FP-II, and HT in 3 h. The different enzymatic action in the current study can be a result of the storage time (the HT and FPII have been stored at −eighty °C whereas Alcalase was saved at 5 °C According to producer advice) and proteases could possibly be auto-hydrolyzed.
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